Part:BBa_K1641008

Fusion protein of Dre::EGFP::ssra-tag, with RBS at beginning

This is a sequence of RBS (BBa_B0034) followed by a fusion protein of Dre::EGFP::ssra-tag. The part is for expression of Dre::EGFP::ssra-tag, which aims to overturn our invertase modules in our Micro-timer.

Dre (BBa_K1641002) in our bricks is a Cre-like recombinase with invertase activity that turns the sequence between two anti-parallel VloxP sites up-side-down. An EGFP fusion at the C-term of Dre will not significantly interfere the enzymatic activity, and a flexible chain of 8 AA is added between the two parts to further avoid side-effect like misfolding. An ssra-tag (BBa_M0051) can accelerate the degradation of the protein, in order to clean it up when not in need and reduce the leakage expression.

Note: The brick sequence starts directly after the XbaI of prefix and ends before SpeI of the Suffix, without the useless bases (G and T respectively) added before and after brick sequence. This deletion do not interfere the usage of the brick.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]